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Immunoblot assays Protein lysates were lysed in mammalian lysis buffer (25 mM Tris-HCl, pH 7.8, 150 mM NaCl, 0.1% NP-40, 1 mM EDTA, 10% glycerol) supplemented with Xpert Protease Inhibitor Cocktail Solution (GenDEPOT, P3100-001)
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