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The transfection of siRNA to cancer cells was performed at concentrations of 50 nM using Lipofectamine RNAiMAX (Life Technologies) in serum-free medium Opti-MEM (Thermo Fisher Scientific) for 5 h
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Impaired insulin-stimulated phosphorylation of Akt and AS160 in skeletal muscle of women with polycystic ovary syndrome is reversed by pioglitazone treatment
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