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Features of Glutathione Agarose: β’ High capacity βbinds at least 40 mg of purified recombinant GST protein per milliliter of resin β’ High yield and purity βconsistently purifies at least 10 mg of GST-tagged protein per milliliter of resin with greater than 90% purity β’ Cost-effective βresin is economically priced and can be reused at least five times without reduction in binding capacity and purification performance β’ Versatile βworks well to purify GST-fusion proteins from bacterial lysates or use with pre-purified GST-tagged proteins to pull down protein interactions β’ Compatible βvalidated and effective for use with Thermo Scientific Cell Lysis Reagents to extract and purify from bacterial or mammalian cell cultures The glutathione is immobilized through its central sulfhydryl onto 6% crosslinked agarose resin
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Radical-trapping antioxidants ferrostatin 1 and liproxstatin 1 are useful in experimental studies to suppress ferroptosis [52]
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