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Analysis of viruses from plasma and from cell culture supernatant indicated that enveloped particles are icosahedral and 56-65 nm[14,15] in diameter, while and the viral core is about 45 nm[14,15]

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This culture was then diluted into 30 mL of YPD medium to give an initial OD 600 of 1.0 and incubated at 30 °C in the rotary shaker for 4 h