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Approximately 200 mg of this tissue powder was transferred into microcentrifuge tubes filled with 1 ml of potassium phosphate buffer (50 mM potassium phosphate (pH 6.8), 0.1 mM ethylenediaminetetraacetic (EDTA), 1 mM phenylmethylsulfonyl fluoride, and 2% (wt/vol) polyvinylpolypyrrolidone (PVPP)
and silicon dioxide
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