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TCA was removed with organic solvents (three volumes of 1,1,2-trichloro-1,2,2-trifluroethane: one volume of trioctylamine) in a ratio of two volumes of organic solvent to one volume of sample

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5-amino-1MQ, an analogue from our initial series of NNMT inhibitors with low IC 50 value (IC 50 = ~1 µM;[17]), and high cell membrane permeability (Table 2), produced the greatest reduction of intracellular 1-MNA levels at a concentration of 10 µM among tested inhibitors (Figure 2D)