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After treatment with 1 µM isopropyl-ß-D-thiogalactoside (IPTG) for 4 h at 30 °C and shaking at 200 rpm, the induced recombinant GST-RILP-C33 and GST proteins were centrifuged at 4 °C for 30 minutes at 5000 xg, with the supernatant discarded and pellet resuspended in 1X bacterial lysis buffer (50 mM Tris, 150 mM NaCl, 10 mM β-mercaptoethanol, pH 7.5 with protease inhibitor cocktail (Sigma-Aldrich)

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