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Nevertheless, we found that a small fraction (~10%) of condensed, ciprofloxacin-treated cells briefly became turgid, as indicated in phase-contrast by phase-light regions becoming phase-dark and increases in cell size, so that hypoosmotic shock appeared to transiently reverse cytoplasmic condensation in these cells (Fig

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The plates were incubated at 37 °C, 5% CO 2 for 3–4 weeks and checked every 2–3 days for colony formation