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After centrifugation at 27,000 g for 20 min at 4 °C, the soluble lysate was incubated with NiNTA resin (Complete His-Tag Purification Resin, Roche) equilibrated with buffer B (20 mM Tris-HCl pH 8.0, 1 M NaCl, 10% glycerol, 0.1% β-mercaptoethanol, 0.2% Triton X-100, 20 mM imidazole, and 1 mM PMSF) for 2 h with end-over-end mixing at 4 °C

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Hyaluronic Acid-Based Injections Hyaluronic acid-based skin boosters are injected directly into the papillary dermis to enhance the skin’s hydration, elasticity, and radiance

Both of them are effective at promoting the burning of fatty acids for energy production and being efficiently used by cells