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Autoimmune disease can cause inflammation that damages joints, muscles, nerves, and connective tissue, leading to chronic pain

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Next, sections probed with primary antibody against ACTL6A (1:400, Abcam, ab3882), GCLC (1:200, Abcam, ab190685), Ki67 (1:400, Cell Signaling Technology, 9449) or 4-HNE (1:4000, Abcam, ab46545) at 4 °C overnight