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The synthesis of GSH from glutamate, cysteine, and glycine involves two steps, catalyzed by glutamate cysteine ligase (also referred to as γ-glutamylcysteine synthetase) and GSH synthetase, respectively, as shown below: 1
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To investigate this, we generated an in vitro pyroptotic model with LPS/ATP stimulated RAW264.7 cells to examine NLRP3 responses to H 2 S [35]
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