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After cleaning the plate for two hours with wash buffer, 100 µL of each sample was added to each well, and the plate was then allowed to incubate at room temperature for an additional two hours
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Size and Gauge:30 Gauge, 0.3 mL, 1/2-inch
Some researchers use a small thermometer inside the fridge to monitor actual temperatures, because the dial setting and the actual internal temperature do not always match
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