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However, in a much larger randomized controlled trial of simvastatin and an antioxidant combination of 600 mg of all - rac -α-tocopherol (297 mg of RRR -α-tocopherol), 250 mg of vitamin C, and 20 mg of β-carotene daily) in more than 20,000 men and women with CHD or diabetes mellitus, the antioxidant combination did not adversely affect the cardioprotective effects of simvastatin therapy over a five-year period (131)

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The DNA obtained was extracted and purified with Qiagen QIAquick PCR purification kit and quantification of immunoprecipitate-enriched DNA sequences was performed by real-time PCR