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The competitive ELISA assays were based upon two main analytical steps: a first step on which analytes were chemically derivatized and a subsequent step enabling the immune-enzyme determination of the derivatized analyte amounts
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Glutamine activates mTORC1 through a special mechanism that requires ADP ribosylation factor 1 and is independent of Rag GTPase to stimulate the lysosomal localization of mTORC1 and to exert its physiological functions, such as controlling cell growth, metabolism and autophagy (35)
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