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Real-Time qPCR Total RNA was extracted from lung tissues using an RNAeasy™ Animal RNA Isolation Kit with a Spin Column (Beyotime Biotechnology, Shanghai, China) and reverse-transcribed to cDNA using a FastKing cDNA First Strand Synthesis Kit (Tiangen, Beijing, China)

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We agree that if additional specialties were added to the model, we would need to consider whether other methodological adjustments were necessary to ensure appropriate comparison groups