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Among the environmental factors involved in the pathogenesis of ASD, prenatal exposure to the antiepileptic drug valproic acid (VPA) has been repeatedly associated with increased risk of neurodevelopmental delay and autistic symptoms in the offspring ( Although the mechanisms by which VPA interferes with development causing autistic-like features in the exposed offspring need to be still elucidated, an imbalance between GABAergic and glutamatergic neurotransmission has been reported, which is also implicated in ASD etiology ( Increased oxidative stress has been identified as a possible mechanism through which environmental factors exert their deleterious effects on the developing brain ( N-acetylcysteine (NAC) is a synthetic derivative of the endogenous amino acid L-cysteine that is able to modulate glutamatergic neurotransmission and the excitatory/inhibitory ratio ( The antioxidant properties of NAC and its ability to modulate glutamatergic neurotransmitters have prompted studies focused on the potential clinical application of NAC in ASD ( Here, we explored the impact of repeated administration of NAC on core autistic-like features (such as social impairments and stereotyped behaviors) in the VPA rat model of ASD

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