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Mitochondrial pellets were suspended in buffer D (1 M 6-amiohexanoic acid, 50 mM Bis-Tris-HCl, pH 7.0), and the membrane proteins were solubilized with digitonin (4 g/g) and incubated for 5 min in ice
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10.1371/journal.pgen.1005158 PLoS Genet
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