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Further analysis revealed that the vanT gene is part of the alanine racemase family, essential for LAB growth (Hols et al., 1997), and not associated with the vanG cluster of vancomycin resistance genes ( vanU, vanR, vanS, vanXY ) (Depardieu et al., 2003)
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The protein concentration was measured and an aliquot (15.6 μg protein) was subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the fractionated proteins were then transferred to a nitrocellulose membrane
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