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For reducing SDS-PAGE, 2 Β΅g of protein was mixed with 3 Β΅L of 4Γ Bolt LDS Sample Buffer (Invitrogen), 1.2 Β΅L of 10Γ Bolt Sample Reducing Agent (Invitrogen), and Milli-Q water to a total volume of 12 Β΅L, and the samples were incubated for 10 min at 95 Β°C
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Vitamin B6 plays a role in converting glutamate to GABA, an inhibitory neurotransmitter, thus maintaining neurotransmitter balance[226]
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